ABSTRACT
BACKGROUND Despite treatment with effective antimalarial drugs, the mortality rate is still high in severe cases of the disease, highlighting the need to find adjunct therapies that can inhibit the adhesion of Plasmodium falciparum-infected erythrocytes (Pf-iEs). OBJECTIVES In this context, we evaluated a new heparan sulfate (HS) from Nodipecten nodosus for antimalarial activity and inhibition of P. falciparum cytoadhesion and rosetting. METHODS Parasite inhibition was measured by SYBR green using a cytometer. HS was assessed in rosetting and cytoadhesion assays under static and flow conditions using Chinese hamster ovary (CHO) and human lymphatic endothelial cell (HLEC) cells expressing intercellular adhesion molecule-1 (ICAM1) and chondroitin sulfate A (CSA), respectively. FINDINGS This HS inhibited merozoite invasion similar to heparin. Moreover, mollusk HS decreased cytoadherence of P. falciparum to CSA and ICAM-1 on the surface of endothelial cells under static and flow conditions. In addition, this glycan efficiently disrupted rosettes. CONCLUSIONS These findings support a potential use for mollusk HS as adjunct therapy for severe malaria.
Subject(s)
Plasmodium falciparum , Malaria, Falciparum , Receptors, Cytoadhesin , Heparitin Sulfate , MolluscaABSTRACT
O consumo de verduras in natura pode ser um meio de transmissão de vários parasitos. Este estudo teve como objetivo a avaliação parasitológica de amostras de verduras comercializadas em supermercados e feiras-livres no município de Umuarama PR. Um total de 48 amostras de alface, 40 de couve, 40 de almeirão e 20 de rúcula foram coletadas, com um total de 20 (13,5%) de positivos para Toxocara (5,4%), Ascaris (5,4%), Hymenolepis nana (1,4%), Entamoeba coli (0,7%) e Taenia (0,7%)...
The consumption of fresh vegetables can be a means of transmission of several parasites. This study aims to assess the presence of enteroparasites in samples of vegetables sold in supermarkets and street markets in the city of Umuarama-PR, Brazil. A total of 48 samples of lettuce, 40 samples of cabbage, 40 of curly endive and 20 of rucola were collected, with 20 (13.5%) being positive, for Toxocara (5.4%), for Ascaris (5.4%), for Hymenolepis nana (1.4%), for Entamoeba coli (0.7%) and for Taenia (0.7%)...
El consumo de verduras in natura puede ser un medio de transmisión de varios parásitos. Este estudio ha tenido como objetivo la evaluación parasitológica de muestras de verduras comercializadas en supermercados y ferias libres en el municipio de Umuarama-PR. Un total de 48 muestras de lechuga, 40 de col, 40 de achicoria y 20 de rúcala, han sido recogidas, con un total de 20 (13,5%) de positivos para Toxocara (5,4%), Ascaris (5,4%), Hymenolepis nana (1,4%), Entamoeba coli (0,7%) y Taenia (0,7%)...